DOF may stand for:
A doffer is someone who removes ("doffs") bobbins, pirns or spindles holding spun fiber such as cotton or wool from a spinning frame and replaces them with empty ones.
Dofetilide is a class III antiarrhythmic agent. It is marketed under the trade name Tikosyn by Pfizer, and is available in the United States in capsules containing 125, 250, and 500 µg of dofetilide.
Doffcocker is a mostly residential district of Bolton, Greater Manchester, lying about 3½ miles from the town centre on the northwest edge of the suburbs on the lower south facing slopes of the West Pennine Moors.
Dofasco is a steel company based in Hamilton, Ontario, Canada. Dofasco is currently a standalone subsidiary of ArcelorMittal, the world's largest steel producer.
Dofus is a Flash-based tactical turn-oriented massively multiplayer online role-playing game (MMORPG) developed and published by Ankama Games, a French computer game manufacturer.
DOF ASA is a Norwegian supply shipping company.
Doftana was a Romanian prison, sometimes referred to as "the Romanian Bastille". Built in 1895 in connection with the nearby mines, from 1921 it began to be used to detain political prisoners, among them Gheorghe Gheorghiu-Dej, who was the Prime Minister of Romania (1952-1955), and the Chairman of the State Council of Romania (1961-1965), and Nicolae Ceaușescu, who was General Secretary of Romanian Communist Party (1965-1989), and the first President of Romania (1968-1989).
Dofus Arena (also known as "Arena Confrontation") was a free turn-based strategic online game and was the second game of Ankama Games.
A doffing cylinder, also called doffing roller or commonly just doffer is a component used in textile mills to remove fiber from the main cylinder of a card, on which the fibers have been straightened and aligned.