DOF may stand for:
A doffer is someone who removes ("doffs") bobbins, pirns or spindles holding spun fiber such as cotton or wool from a spinning frame and replaces them with empty ones.
Dofetilide is a class III antiarrhythmic agent. It is marketed under the trade name Tikosyn by Pfizer, and is available in the United States in capsules containing 125, 250, and 500 µg of dofetilide.
Dofasco is a steel company based in Hamilton, Ontario, Canada. Dofasco is currently a standalone subsidiary of ArcelorMittal, the world's largest steel producer.
Dofus is a Flash-based tactical turn-oriented massively multiplayer online role-playing game (MMORPG) developed and published by Ankama Games, a French computer game manufacturer.
Dofus Arena (also known as "Arena Confrontation") was a free turn-based strategic online game and was the second game of Ankama Games.
Doffing is a census-designated place (CDP) in Hidalgo County, Texas. The population was 5,091 at the 2010 United States Census.
Dofleinia armata, common names striped anemone and armed anemone, is a species of sea anemone in the family Actiniidae.
The Dofflemyer Point Lighthouse is a U.S. lighthouse in Boston Harbor, Washington, and was one of the first in the state of Washington to be automated.
A doffing cylinder, also called doffing roller or commonly just doffer is a component used in textile mills to remove fiber from the main cylinder of a card, on which the fibers have been straightened and aligned.