DOF may stand for:
A doffer is someone who removes ("doffs") bobbins, pirns or spindles holding spun fiber such as cotton or wool from a spinning frame and replaces them with empty ones.
Dofasco is a steel company based in Hamilton, Ontario, Canada. Dofasco is currently a standalone subsidiary of ArcelorMittal, the world's largest steel producer.
Dofus Arena (also known as "Arena Confrontation") was a free turn-based strategic online game and was the second game of Ankama Games.
Doffing is a census-designated place (CDP) in Hidalgo County, Texas. The population was 5,091 at the 2010 United States Census.
The Dofflemyer Point Lighthouse is a U.S. lighthouse in Boston Harbor, Washington, and was one of the first in the state of Washington to be automated.
A doffing cylinder, also called doffing roller or commonly just doffer is a component used in textile mills to remove fiber from the main cylinder of a card, on which the fibers have been straightened and aligned.